Journal: Neuron

Article Title: The age of olfactory bulb neurons in humans.

doi: 10.1016/j.neuron.2012.03.030

Figure Lengend Snippet: Figure 2. Identification and Isolation of Neuronal Nuclei in the Human Olfactory Bulb (A and B) Nuclei were labeled with isotype control antibody (A) or with antibodies to NeuN/Fox3 (B). (C) Antibodies to NeuN label most neurons (arrows), but not large neurons in the mitral/gran- ular layer. (D and E) Nuclei were labeled with iso- type control antibody (D) and neuronal nuclei were identified by the presence of HuD and the absence of Sox10 (E). (F) HuD antibodies label mitral cells (asterisk), other neurons (arrows), and a subset of oligodendrocyte lineage cells identified by Sox10 and CNPase expression (arrowheads). Arrows indicate NeuN+ (C) or HuD+/Sox10 (F) neuronal nuclei. The sorting gates for neuronal nuclei are indicated in (B) and (E). SSC, side scatter. Scale bars indicate 10 mm.

Article Snippet: Sections were incubated with the respective primary antibody overnight at 4 C: mouse NeuN (Millipore A-60 clone; 1:100), rabbit Fox3 (Atlas Antibody, 1:300), goat Sox10 (R&D, 1:100), rabbit calbindin (Abcam, 1:200), chicken MAP-2 (Abcam, 1:1,000), rabbit calretinin (Abcam, 1:200), rabbit parvalbumin (Abcam, 1:1,000), rabbit tyrosine hydroxylase (TH) (Millipore, 1:1,000), rabbit GAD65/67 (Millipore, 1:500), rabbit CNPase (Atlas Antibody, 1:400), mouse GFAP (Sigma Aldrich, 1:1,000), rabbit Iba1 (Wako, 1:1,000), mouse HuD (E-1) (Santa Cruz, 1:100), and mouse HuD/HuC 16A11-biotin (Invitrogen, 1:100), and visualized with the matching secondary antibody and streptavidin conjugated to Alexa 488, 546, or 647 (1:1,000, Invitrogen).

Techniques: Isolation, Labeling, Control, Expressing

Journal: STAR Protocols

Article Title: Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice

doi: 10.1016/j.xpro.2024.103322

Figure Lengend Snippet:

Article Snippet: Chicken anti-Fox3 (NeuN) (1:500) , Aves Labs , NUN-0020.

Techniques: Plasmid Preparation, Recombinant, Sterility, Saline, Electron Microscopy, RNAscope, Multiplex Assay, Microscopy

Journal: Cell Reports Methods

Article Title: A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes

doi: 10.1016/j.crmeth.2022.100276

Figure Lengend Snippet:

Article Snippet: Chicken polyclonal anti-Foxo3 , EnCor , Cat#CPCA-FOX3; RRID: AB_2747400.

Techniques: Virus, Recombinant, Plasmid Preparation, RNAscope, Multiplex Assay